DNA+exp+group2


 * Preparing the agarose gel **
 * 1) The gel is already prepared and we about 10 ml of molten agarose into the gel box so that it fills the central cavity and flows under and between the teeth of the comb. **__Note:__** avoid spilling liquid in to the areas of either end.
 * 2) Leave the box and let is set hard.
 * 3) Cut 2 pieces of the carbon fibre tissue (about 42mm x 22mm). These will act as electrodes at either end of the gel box.
 * 4) Place the electrodes to one side until you have loaded the gel into the wells.
 * Loading the gel **
 * 1) Pour slightly more then 10 ml of buffer solution into the gel box. The liquid should just cover the surface of the gel and flood into the areas that will hold the electrodes.
 * 2) Ease the comb gently from the gel allowing the buffer solution to fill the wells left behind. Note: do not tear the wells
 * 3) Leave the gel box undisturbed
 * 4) Put a clean tip on the microsyringe
 * 5) Add 2 microlitres of the dye to the tube containing the DNA.
 * 6) Mix the dye and DNA sample thoroughly.
 * 7) Take a sample with the pipette of the loading dye and place it above the well but under the buffer solution. **__Note:__** do not puncture the bottom of the well with the tip of the microsyringe.
 * 8) Make a note of which DNA you have put into each well.
 * 9) Repeat the steps 4-8 with each DNA and the “control” tube with not restriction enzymes.
 * Running the gel **
 * 1) Place one electrode at each end of the gel box
 * 2) Join the electrodes to the batteries using wires with alligator clips. **__Note:__** ensure that the positive of the battery is connected to the negative electrode.
 * 3) Check that contact is made between the buffer solution and the electrodes.
 * 4) leave the gel to run for 3.5 hours
 * 5) After 3.5 hours have past disconnect the batteries once you have noticed the blue loading dye has reached the end of the gel.
 * 6) Rinse the alligator clips in water and dry them.
 * __ Caution: __**
 * Never pull the plunger right out of the microsyringe
 * Never cross contaminate always use a clean tip on the microsyringe
 * Wear gloves! Wear **__new__** gloves when using different DNA

LOVE Elena "Arkadivnooch" Benski, Gina "crying or laughing?" Reichenberg, Simone "SB" Starikov.