DNA+exp+group3

After reading through the experiment booklet, create an easy to follow running sheet (dot point) of the key steps involved. You might also want to include some important notes on safety or errors to avoid. This must be done by Friday morning before class.

Link back to DNA manipulation SAC page

Step 1 – Using the mircrosringes (already done) Step 2 – Rehydrating the dried lambda DNA (10 – 15 minutes) - adding distilled water to DNA to become a solution - use gloves Step 3 and 4 – Cutting DNA with restriction enzymes (approx. 60 minutes) - adding restriction enzymes to cut the DNA - ensure that it is labelled - put into incubator - use gloves Step 5 – Preparing the agarose gel (20 – 40 min) - ensure the comb is correctly inserted into the gel to create the wells - prepare agarose while the DNA is in the incubator with the restriction enzymes Step 6 – Loading the gel (10 – 15 minutes) - buffer solution - take away the comb gently - add DNA to the wells and mark which restriction enzyme it contains - use gloves Step 7 – Running the gel (5 min) - ensure that the electrodes are corrected to the correct terminals
 * Make sure that we have all equipment needed before proceeding **
 * Do everything together ****J **