biol+4+manipulation+of+DNA+SAC

This SAC is about DNA technology and will relate to an experiment you will conduct in class.

Link to gel electrophoresis page Back to biol 4 SACs page

Timeline of events:
 * ** Tuesday 8th September ** || ** revison of DNA technology and info about experiment given out ** ||
 * ** Friday 11th September ** || **experiment in class** ||
 * ** Monday 14th September ** || ** examining results of experiment ** ||
 * ** Tuesday 15th September ** || ** written report SAC in class ** ||
 * Gene technologies is a large area, this SAC is not going to be about all of them. The concepts you do need to know are: **

**// Molecular tools and techniques //**
 * restriction enzymes, gel electrophoresis, PCR, recombinant DNA**


 * // Applications //**
 * DNA profiling, detecting mutations, transforming bacteria**

As you can see from the criteria, most if it will be about the experiment, so know the procedure well and be able to relate it to any relevant theory.

Q. VOJ, do we need to know about FISH (Fluorescent In Situ Hybridization) for the upcoming SAC? "Bubbly" // A. Nope, the only things you need to worry about for this SAC are those terms I mentioned above. VM //

Q. What is DNA fragmentation? :) // A. Hmmm.. not sure where you came across this term but DNA fragmentation refers to the breakdown or breaking apart of DNA (as in apoptosis). We also refer to DNA fragments as the products due to cutting with restriction enzymes. VM //

Q.Hi Voj. In regards to DNA transcriptase, do we need to know about micro array shotgun method of sequencing, as well as DNA sequencing? Thanks, LB // A. As you can see from above (I have now highlighted them a different colour!), I have mentioned the areas that you need to focus on, that is all I will say. VM //

Hi Voj I thought this was a pretty good video http://www.youtube.com/watch?v=x2jUMG2E-ic .. although no sound.. but it explains the different types of recombinant DNA, however not it's uses.... so my question: Q. What are the different uses for recombinant DNA and do we need to know them? // A. As you can see above, I have mentioned recombinant DNA. We can get bacteria to clone human genes or any gene for that matter. We can insert foreign genes into plants to give them traits they normally wouldn't have. These would be the two main uses of recombinant DNA. And yes, I expect you to know what these involve and so will VCAA. VM //

Choose a group no and create a group name if you wish and list your group members (initials only). ** Hmmm... maybe I'll ask for full names and then I will get your intials. ** Complete your running sheets for Friday's experiment on your particular page.
 * I wonder if Joshua Meir Appelboom in group 1 is related to Josh "edge" Appelboom in group 4. Could very well be. **


 * = ** Group no. ** ||= ** Group name ** ||= ** Group members ** ||= ** Link to your experiment page ** ||
 * = **1** ||= Teenage Mutant Biol Students ||= Mikayla jadique Greenwald, Olivia Sarah Sandler, and ???? ||= DNA exp group1 ||
 * = **2** ||=  ||= GR, EB AND SS ||= DNA exp group 2 ||
 * = **3** ||=  ||= Yossi, Bec and Adam :) ||= DNA exp group 3 ||
 * = **4** ||=  ||= Josh "Edge" Appelboom, Lauren "Curtis Mayfield" Basser and Sarah "Insert Palendrome" Bush ||= DNA exp group 4 ||
 * = **5** ||=  ||=   ||= DNA exp group 5 ||

Vojtech ive written a running sheet but not really sure if it includes everything at the right time because i know there is a lot of simultaneous stuff that happens during the experiment. Can you do a bit of guiding tomorrow? and maybe you can clear one thing up for me. Is the dry DNA already with each of the dry enzymes in a separate tube i.e one tube with dna and one enzyme, another tube with the dna and another enzyme etc. then we just turn it into a solutoin by adding the water done via pipetting? (also, check out the band paffendorf - they're awesome) Edge You needn't fear about tomorrow. My role is to assist you all in the procedure. It's not like I am going to just sit back and watch you fumble your way through it.So, I will clear up everyone's concerns as you are working your way through the experiment. The aim of the running sheet was to get you to think about the prac before you do it. VM